Human DeltaSIP-Alpha (Delta Sleep Inducing Peptide Alpha) ELISA Kit

Human DeltaSIP-Alpha (Delta Sleep Inducing Peptide Alpha) ELISA KitThe Human DeltaSIP Alpha (Delta Sleep Inducing Peptide Alpha) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human DeltaSIP Alpha. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Human DeltaSIP Alpha. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and

The Human DeltaSIP-Alpha (Delta Sleep Inducing Peptide Alpha) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human DeltaSIP-Alpha. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Human DeltaSIP-Alpha. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DeltaSIP-Alpha in the samples is then determined by comparing the OD of the samples to the standard curve.